Molecular biology

DNA Isolation Techniques

Genomic DNA isolation from various sources: This technique allows us to extract the complete genetic blueprint (genome) from microorganisms, plants, and animals.

Plasmid DNA isolation: Plasmids are small, circular DNA molecules found in bacteria. Isolating plasmid DNA is crucial for various applications, such as gene cloning and protein expression.

RNA Analysis Techniques

RNA isolation: This technique isolates RNA (ribonucleic acid), the essential molecule for protein synthesis.

cDNA synthesis: After RNA isolation, Weevil Biotech may use reverse transcription (RT) to convert RNA into complementary DNA (cDNA) for further analysis or manipulation.

DNA Amplification and Analysis Techniques

Specific amplification of DNA by PCR (Polymerase Chain Reaction): PCR is a powerful technique that allows for the targeted amplification of specific DNA sequences in millions of copies. This is crucial for various applications, including diagnostics and gene cloning.

Advanced PCR Techniques: Weevil Biotech utilizes various advanced PCR techniques like RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA), LAMP PCR (Loop-mediated isothermal amplification PCR), Nested PCR (amplifying a specific region within a previously amplified region), Multiplex PCR (amplifying multiple targets simultaneously), and RT-PCR (reverse transcriptase PCR for amplifying cDNA).

qPCR (quantitative PCR): This advanced PCR technique allows for the quantification of specific DNA targets, providing valuable insights into gene expression levels.

Analysis Techniques

Agarose gel electrophoresis: This technique separates DNA fragments based on their size, allowing for visualization and analysis.

Additional Techniques

DNA Fragmentation Assays: These assays may be used to break down DNA into specific sized fragments for further analysis or manipulation.

Primer and Probe Design: Weevil Biotech designs specific primers and probes for various applications, including real-time PCR and gene expression studies using qPCR.

Molecular Cloning, Expression, and Purification: These techniques are crucial for producing and isolating desired proteins for various biotechnologies. Weevil Biotech may utilize column chromatography as one of the purification methods.