DNA Isolation Molecular Biology Techniques
Genomic DNA isolation from various sources: This technique allows us to extract the complete genetic blueprint (genome) from microorganisms, plants, and animals.
Plasmid DNA isolation: Plasmids are small, circular DNA molecules found in bacteria. Isolating plasmid DNA is crucial for various applications, such as gene cloning and protein expression.
RNA Analysis Techniques
RNA isolation: This technique isolates RNA (ribonucleic acid), the essential molecule for protein synthesis.
cDNA synthesis: After RNA isolation, Weevil Biotech may use reverse transcription (RT) to convert RNA into complementary DNA (cDNA) for further analysis or manipulation.
DNA Amplification and Analysis Techniques
Specific amplification of DNA by PCR (Polymerase Chain Reaction): PCR is a powerful technique that allows for the targeted amplification of specific DNA sequences in millions of copies. This is crucial for various applications, including diagnostics and gene cloning.
Advanced PCR Techniques: Weevil Biotech utilizes various advanced PCR techniques like RFLP (Restriction Fragment Length Polymorphism), RAPD (Random Amplified Polymorphic DNA), LAMP PCR (Loop-mediated isothermal amplification PCR), Nested PCR (amplifying a specific region within a previously amplified region), Multiplex PCR (amplifying multiple targets simultaneously), and RT-PCR (reverse transcriptase PCR for amplifying cDNA).
qPCR (quantitative PCR): This advanced PCR technique allows for the quantification of specific DNA targets, providing valuable insights into gene expression levels.
Analysis Techniques
Agarose gel electrophoresis: This technique separates DNA fragments based on their size, allowing for visualization and analysis.
Additional Techniques
DNA Fragmentation Assays: These assays may be used to break down DNA into specific sized fragments for further analysis or manipulation.
Primer and Probe Design: Weevil Biotech designs specific primers and probes for various applications, including real-time PCR and gene expression studies using qPCR.
Molecular Cloning, Expression, and Purification: These techniques are crucial for producing and isolating desired proteins for various biotechnologies. Weevil Biotech may utilize column chromatography as one of the purification methods
Isolation and Identification: We isolate bacteria from various sources like soil, water, and food, employing traditional and molecular methods for their precise identification.
Biochemical and Molecular Characterization:
Through a combination of enzyme assays and DNA sequencing, we unravel the metabolic capabilities and unique characteristics of these bacteria.
Antimicrobial Activity Analysis: We explore the potential of bacteria to combat harmful pathogens, searching for natural alternatives to antibiotics and antifungal agents.
Susceptibility Testing: We determine the effectiveness of existing antibiotics against various bacterial strains, aiding in the development of targeted treatment strategies.
Enzymatic Assays: We assess the production of key enzymes like amylase, lipase, and protease, identifying bacteria with potential applications in industry and environmental cleanup.
Media and Growth Optimization: We optimize growth conditions such as media composition, pH, and temperature to maximize the production of desired enzymes from specific bacteria.
Secondary Metabolite Screening: We delve into the world of secondary metabolites produced by bacteria, potentially leading to the discovery of novel bioactive compounds for medicine, agriculture, or industrial applications.
Bacterial Agglutination Studies: We investigate the ability of antibodies to clump bacteria, a technique with potential applications in diagnostics.
Antagonistic Activity Exploration: We explore the potential of beneficial bacteria to suppress harmful fungi, promoting natural and sustainable solutions in agriculture.
Plastic Degradation Investigation: We isolate and characterize bacteria with the ability to degrade plastic waste, contributing to a cleaner environment.
Bioremediation Efficacy Analysis: We assess the effectiveness of microorganisms in bioremediation efforts, a valuable tool for cleaning up contaminated environments.
Probiotic Isolation and Characterization: We isolate and characterize probiotic bacteria, promoting gut health and overall well-being.
microbial testing of water and food samples
At Weevil Biotech, we are committed to ensuring the safety and quality of your food and water. Our state-of-the-art testing facilities employ rigorous microbial testing methods to identify potential contaminants and guarantee a healthy environment for everyone.
Our Testing Services:
Total Plate Count (TPC): This essential test determines the overall bacterial and fungal population in a sample, providing a general measure of hygiene. High TPC levels may indicate improper handling or processing.
Total Coliforms: The presence of these bacteria suggests potential fecal contamination and the possible presence of harmful pathogens. Early detection allows for corrective actions to prevent outbreaks of foodborne illness.
Total Yeast/Mold Count: Yeasts and molds can spoil food products, reduce shelf life, and trigger allergic reactions in sensitive individuals. Our testing helps ensure the quality and safety of your food.
Identification of Specific Pathogens: We test for a range of pathogenic bacteria, including E.coli, Salmonella spp., Staphylococcus aureus, Pseudomonas aeruginosa, and Shigella spp. These bacteria can cause severe foodborne illnesses, and their early detection is crucial for ensuring food safety.
Most Probable Number (MPN): When the levels of specific bacteria are too low for direct detection by TPC methods, the MPN technique offers a reliable way to estimate their concentration in water or food samples.
Proteins are the building blocks of life, playing essential roles in virtually every cellular process. At Weevil Biotech, we offer comprehensive protein services to support your research and development needs. From extraction to characterization, our team of experts utilizes cutting-edge techniques to unlock the secrets of these vital molecules.
Protein Extraction: We utilize efficient methods to isolate proteins from diverse biological samples, such as tissues, cells, and bacteria.
Protein Quantification: Accurate protein quantification is crucial for various downstream applications. We employ reliable methods to determine protein concentration in your samples.
Protein Isolation by Column Chromatography: Techniques like ion exchange chromatography and affinity chromatography allow us to purify specific proteins of interest for further analysis.
Protein Characterization: We utilize various techniques to characterize the properties of purified proteins, including size, structure, and function.
Protein Analysis by Gel Electrophoresis:
Native PAGE: This technique separates proteins based on their size and shape, offering valuable insights into protein complexes.
SDS-PAGE: This widely used technique separates proteins based on their size alone, providing a detailed profile of protein components in a sample.
Protein Visualization: Techniques like silver staining and Coomassie Brilliant Blue staining visualize protein bands separated by gel electrophoresis, allowing for protein identification and quantification.
MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight): This advanced technique allows for rapid and accurate identification of proteins by analyzing their mass fingerprint.
At Weevil Biotech, we utilize the transformative power of biotechnology to address global challenges and cultivate a healthier future for all. We offer a comprehensive range of services that empower researchers, developers, and industries to unlock the immense potential of living organisms.
Plant Tissue Culture:
Micropropagation: We excel in multiplying plants efficiently through micropropagation techniques. This allows for rapid production of disease-resistant plants, preservation of endangered species, and generation of valuable plant metabolites for diverse applications.
Genetic Improvement: We explore the potential of tissue culture for targeted genetic improvements in plants, potentially leading to increased yields, enhanced nutritional value, and improved stress tolerance.
Animal Tissue Culture:
Cell Line Development: We establish and maintain stable animal cell lines for various purposes, including vaccine production, drug discovery, and research on cellular functions.
Cryopreservation: We utilize cryopreservation techniques to preserve valuable animal cell lines for future use, ensuring long-term viability and accessibility.
Development of Monoclonal Antibodies:
Tailored Solutions: We develop highly specific monoclonal antibodies for a range of applications, including diagnostics, targeted therapies, and bioremediation strategies.
Expertise: Our team of immunologists possesses extensive experience in designing and producing high-affinity monoclonal antibodies to meet your specific needs.
DNA Cloning:
Advanced Techniques: While DNA cloning is a fundamental step in biotechnology, Weevil Biotech goes beyond the basics. We utilize advanced techniques for efficient gene isolation, vector selection and preparation, and transformation.
Expression and Purification: Our expertise extends to expressing and purifying recombinant proteins encoded by the cloned genes, paving the way for functional studies and potential applications.
Weevil Biotech offers a comprehensive range of immunology technique services to support your research. Our expert team can assist you with:
Western Blotting: A powerful technique for detecting and identifying specific proteins in a sample. Widely used for protein analysis in various research fields.
Agglutination Test: A method for detecting antigens or antibodies based on their ability to cause clumping (agglutination) of particles. Used in blood typing, diagnosing infectious diseases, and more.
Immunohistochemistry (IHC): A technique used to visualize the location of specific proteins within tissues. Valuable for cancer diagnosis, studying protein expression in tissues, and other applications.
Phytochemical Screening: Our advanced techniques allow us to identify a broad range of bioactive compounds present in plants, including:
Total Phenol, Alkaloid, Flavonoid, Terpenoid, Anthocyanin, Saponin, Total chlorophyll, Total Lipid, Phytosterols, Tannin, Steroids, Anthraquinone, Glycosides, Carbohydrates, Total proteins and aminoacids
Purification of Bioactive Compounds: Once promising compounds are identified, our team utilizes sophisticated methods to isolate and purify them for further study.
Analysis of Therapeutic Potential:
In Vitro Assays: We employ a battery of in vitro assays to assess the potential therapeutic properties of natural compounds. These assays include:
Antioxidant Studies: We evaluate the ability of compounds to scavenge free radicals, potentially offering benefits for various conditions.
Disease-Specific Assays: We offer a variety of assays to target specific diseases, such as:
In Vitro Anti-Inflammatory Studies
In Vitro Anti-Diabetic Studies
In vitro Anti-urolithiatic study
Hemagglutination Studies: This assay helps assess potential interactions with red blood cells, providing valuable insights in the drug discovery process.
In Vitro Antioxidant Enzyme Activities: We measure the activity of enzymes (SOD, CAT, GPx) that combat oxidative stress, a factor in many diseases.
Green or chemical synthesis and characterization of nanoparticles for biomedical / diagnostic applications.
Histopathology study
Blood smearing
Periodic acid-Schiff reaction (PAS for glycoprotein)
Mosquito Identification
Insecticide toxicity analysis
Insect bio assay
Mosquito larvicidal analysis of various compounds
Primer and probe designing
Sequencing
NGS
Genome Analysis
RNA-Seq Analysis
Microbiome Analysis
Drug Discovery by Molecular Docking
Phylogenetic tree
DNA bar-coding and GenBank submission
Analysis of Soil and water quality
Analysis of Seed germination study
Health Management of Fish
Fish Acute Toxicity Test(Zebra fish, Guppy and carp)
Fish, Early-life Stage Toxicity Test
Fish Embryo Acute Toxicity (FET) Test
Algae Growth Inhibition Test
Daphnia Immobilization Test
Earthworm, Acute Toxicity Tests
Honeybees, Acute Oral Toxicity Test
Honeybees, Acute Contact Toxicity Test
Wastewaters analysis: pH, Temperature, EC,COD, BOD, Nitrate, Ammonia,Oil & grease
Solution to waste water treatment
Heavy metal analysis
Soil sample analysis : Macronutrient (NPK), Secondary Nutrients (S, Ca, Mg) Micronutrients (Zn,Cu, Fe,Mn,B, Mo)
GC-MS, LC-MS, HPLC, HPTLC, AAS, FTIR, NMR, HRSEM-TEM, XRD, MALDI-TOF.